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Biophysical Instrumentation Facility

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Overview

The CSB maintains a variety of instruments for studying the conformation and stability of macromolecules and for measuring the affinity and thermodynamics of biomolecular interactions. To set up core access and training, contact聽Noah Saracho. If you have additional questions or would like to set up a consultation, contact聽Heather Kroh.

Services and Fees

  • Find us in iLab
    • The Biophysical Instrumentation Facility and the Megalab Core have been combined to form the new 汤头条 CSB Macromolecular Biophysics Core in iLab. The Biomolecular Crystallography Facility was added in November 2024.
  • Training
    • Required of all first time users
    • Must be completed before self-service access is granted
    • Covers basic operation of the instrument
    • To schedule training*, contact聽Noah Saracho or Heather Kroh and provide an iLab billing number for you or your PI
    • See Fees section below for current training fees
  • Instrument Time
    • Includes access to instrument and accessories as scheduled by user
    • Short-term data file storage
    • Access to LAN and printer for report generation or file transfer
    • Basic operational assistance (initial set-up, instrument malfunction resolution)
    • Maintenance and repair of the instrument and accessories
  • Fees
    • Fees are set yearly by Core Management and subject to change
    • Current training fee for new users of CSB biophysical instruments is $59.80
    • Current Usage Rates:
    • Additional Charges: Damage to equipment caused by user negligence or abuse will result in charges for replacement or repair costs as well revocation of access privileges.

Core Instruments

  • Octet R8 Bio-layer Interferometer

    • Sartorius Octet R8
    • High-quality quantitative, kinetic, or affinity characterization of biomolecules
    • Can run up to 96 samples in a single experiment
    • Installed in January 2023
  • Mass Photometer

    • Reyfeyn TwoMP Mass Photometer
    • Measure molecular mass of proteins and
      protein assemblies in solution in their native state
    • Quick and accurate mass determination with minimal sample requirements (<20 碌L, low nanomolar concentrations)
    • Installed in January 2023
  • Stopped-Flow Spectrometer

    • Kintek SF300X Stopped-flow Spectrometer
    • Measures changes in absorbance and/or fluorescence signals for kinetic and equilibrium titration experiments
    • Minimal first reaction time of 5 millisecond, with a sub-millisecond dead time
    • Computer-controlled servo motor (not air-driven)
    • Capacity for single- and double-mixing experiments
  • Circular Dichroism Spectropolarimeter

    • Chirascan VX CD spectrometer
    • User friendly data acquisition and analysis
    • Peltier temperature control
    • Installed December 2022
    • CD Guidelines
  • Microscale thermophoresis

    • NanoTemper Monolith NT.115
    • Determine binding affinities using fluorescently labeled ligands
    • Equipped with two LED lasers, blue and red
    • MST Guidelines
  • Tycho Sample Quality Check

    • Nanotemper Tycho NT.6
    • Verify protein quality, stability and reproducibility, with minimal volume needed (10 碌L)
    • Measures intrinsic fluorescence of tryptophan and tyrosine residues
    • Can detect impurities, compare concentrations, and screen storage conditions
  • Differential Scanning Calorimeter

  • Dynamic Light Scattering

  • Isothermal Titration Calorimeter

    • TA Instruments Affinity ITC
    • Installed in February 2020
    • Injection Syringe Volume up to 250 碌L
    • Active cell volume of 1.0 mL
    • Operating Temperature Range from 2 掳C to 80 掳C
    • Fully automated Injection Syringe Cleaning
    • ITC Notes

Accessing Data on the Network Drive

CD, fluorometer or calorimeter data saved to network J: drive can be accessed remotely.

  • Windows
    • Right click 鈥楽tart鈥� and then click 鈥楻un鈥�
    • 罢测辫别听袄袄肠别蝉.颈迟.惫补苍诲别谤产颈濒迟.别诲耻袄肠蝉产蝉丑补谤别诲颈苍蝉迟谤
    • Click 鈥極K鈥�
    • It should prompt you to sign in. 罢测辫别听痴础狈顿贰搁叠滨尝罢袄测辞耻谤痴鲍苍别迟滨顿
    • Enter your e-password.
    • Double click on the Data folder.
    • You should see folders for the CD, DSC, Fluoromax and ITC.
  • Mac
    • From the Finder menu, click 鈥楪o鈥� and then click 鈥楥onnect to Server鈥�
    • It should prompt you for the Server Address. Type聽smb://ces.it.vanderbilt.edu/csbsharedinstr
    • Click 鈥楥onnect鈥�
    • It should prompt you for your VUnetID and e-password.
    • Double click on the Data folder.
    • You should see folders for the CD, DSC, Fluoromax and ITC.
  • Linux
    • The easiest way to access it is through the file manager in Gnome:
    • Click 鈥楶laces鈥� and then click 鈥楥onnect to Server鈥�
    • 蝉别谤惫别谤:听肠别蝉.颈迟.惫补苍诲别谤产颈濒迟.别诲耻
    • 蝉丑补谤别:听肠蝉产蝉丑补谤别诲颈苍蝉迟谤
    • 耻蝉别谤:听测辞耻谤痴鲍苍别迟滨顿
    • 诲辞尘补颈苍:听痴础狈顿贰搁叠滨尝罢
    • Click 鈥楥onnect鈥�
    • A window with the contents of your linux home dir may popup, but just close the popup and then click on the new icon for the share on the desktop. It will ask you for your e-password.
    • If you鈥檙e using KDE, then just use the file browser (konqueror or dolphin, e.g. with KDE v4+):
    • Go to聽\\ces.it.vanderbilt.edu\csbsharedinstr
    • 罢测辫别听痴础狈顿贰搁叠滨尝罢袄测辞耻谤痴鲍苍别迟滨顿
    • Enter your e-password.
    • It鈥檚 also possible to mount the share into the filesystem for access via a linux terminal. For more information, see:聽聽and adapt to the server and share above.